Stage 1 (2023) of the research project ”Volatile organic compounds from extracellular vesicles as a source of additional diagnostic information” acronym Volatevs was entitled ”Release of organic volatile compounds from extracellular vesicles”. Various aspects were considered, related with extracellular vesicles, organic volatile compounds that they contain and the possibility to use them as biomarkers in metabolomi and volatolomic analysis. Identification of suitable parameters for the release of the organic volaile compounds from the extracellular vesicles and their concentration using solid phase microextraction fibres for the trasfer to a gas chromatograph coupled with a mass spectrometry detector was also performed.