The main objective will be approached at three levels (specific objectives O1 – O3), starting with obtaining the nanoporous fibers and ending with the validation of the obtained product on experimental animals.

O1. Preparation of three chitosan porous fibers loaded with norfloxacin and sealed with 2-formylphenylboronic acid, denoted as candidate formulations.

            This first objective will be accomplished in the first 12 months of the project implementation, and consists of obtaining and characterization of the candidate formulations.

O2. In vitro evaluation of the efficacy and safety of the candidate formulations. 

                      The second objective of the project is the in vitro evaluation of the performances of the candidate formulations in terms of efficacy and safety (in vivo biocompatibility tests, biodegradability tests, in vitro release of the norfloxacin, antimicrobial activity)

O3. In vivo evaluation of the efficacy and safety of the candidate formulation on burn-induced mouse models.

                  The third objective of the project consists of the investigation of the efficiency and safety of the candidate formulations on mice animal models, as an necessary stage for effectively translating laboratory outcomes to clinical trials. For this objective, the candidate formulations that passed in vitro biocompatibility and bacterial inhibition tests will be selected.

Results' dissemination will be an activity running continously during the implementation of the project.