Stage 1/2024. Synthesis of carboxy- and amino- derivatives of Zn phthalocyanine (ZnPc). Conjugation with noble and magnetic metal nanoparticles (NPs). Characterization by FTIR spectroscopy, NMR, EDX analysis and mass spectroscopy. Biological evaluation
1.1. Optimization of the synthesis of carboxy ZnPc derivatives (tetra, octa) using the condensation and hydrolysis method
1.2. Synthesis of magnetic nanoparticles (Fe3O4) by coprecipitation of Fe2+ and Fe3+ in the presence of NH3•H2O and dispersed in ethanolic solution. Functionalization of nanoparticles with extracts from local microalgae or polysaccharides, peptides, glucosides
1.3. Synthesis of supramolecular architectures (tetra, octa) carboxy ZnPc/functionalized nanoparticles
1.4. Structural characterization of different supramolecular architectures obtained (tetra, octa) carboxy ZnPc/functionalized nanoparticles by FTIR and NMR
1.5. Commercially available noble metal (tetra, octa) carboxy ZnPc architectures anchored with extracts (polysaccharides, peptides, glucosides) from local microalgae
1.6. Determination of the composition of the supramolecular architectures obtained by EDX analysis and mass spectrometry
1.7. Investigation of cell viability and zeta potential
2. Training on absorption and emission spectroscopy methods and techniques: fluorescence and phosphorescence.
Stage 2/2025. Photophysical properties and biological evaluation of the synthetized supramolecular architectures, in solvents with different polarity accepted for pharmaceutical formulations. Synthesis of supramolecular architectures based on SiPc derivatives conjugated with metal nanoparticles (Au, Ag)/metal oxides and characterization with FTIR, NMR spectroscopy. Cytotoxicity evaluation
2.1. Investigation of the UV-Vis absorption of the synthesized supramolecular architectures in aqueous solution. Evaluation of the relationship between the concentration of (tetra-, octa-) carboxy ZnPc derivatives in solution and the intensity of the absorption bands
2.2. Investigation of the effect of the concentration of (tetra-, octa) carboxy ZnPc derivatives in aqueous solutions of supramolecular structures; the effect of pH, solvent on cytotoxicity
2.3. Evaluation of the lifetime, quantum yield of fluorescence and phosphorescence, singlet oxygen yield in the synthesized supramolecular architectures
2.4. Dissemination of results
2.5. Synthesis of octabutoxy phthalocyanines containing axial carboxylic ester groups or diethyl phosphonate via phenoxy groups of different SiPc derivatives using the method of microwave irradiation of the reactant mixture, classical method, etc. Confirmation of the respective structures by FTIR and NMR
2.6. Synthesis of SiPc derivatives obtained by standard methodology, using the reaction of SiPC dichloride with carboxylic acids in polyether solvent and structural characterization by FTIR and NMR
2.7. Synthesis of supramolecular architectures based on SiPc derivatives/noble metal nanoparticles (Au, Ag)
2.8. Synthesis of supramolecular architectures based on SiPc derivatives/metal oxide nanoparticles
2.9. Structural characterization of different supramolecular architectures based on silicon phthalocyanine derivatives/metal oxide nanoparticles by FTIR and NMR
2.10. Determination of the composition of supramolecular architectures obtained by EDX analysis and mass spectrometry
2.11. Cytotoxicity analysis for different cancer cell lines using the MTT and MTS method depending on the concentration, dose and duration of irradiation, nature of the solvent | 
