Stage 2025 Summary
Activities:
A1.1. Extraction of kaempferol from local plant sources
A1.2. Characterization of kaempferol extracts
A1.3. Fabrication of new bionanocomposites. Kaempferol-Deep Eutectic Solvents complexes
Estimated results:
- database and characterization reports for kaempferol extracts;
- database and characterization reports for natural eutectic solvents;
- database and characterization reports for kaempferol complexes with natural eutectic solvents;
- working visits to USMF Moldova and internships at ICMPP Iasi;
- training in the project's research area for doctoral and post-doctoral students;
- project website;
- dissemination of results through participation in national/international conferences;
- 1 ISI article submitted for publication;
- scientific report.
Obtained results:
A1.1.
· Collection of plant raw material (Armoracia rusticana).
· Extraction process carried out according to the procedures set out in the research program.
· Obtaining primary extracts and concentrated extracts for further use.
A1.2.
· Performing preliminary spectroscopic and physicochemical analyses on the extracts obtained.
· Identifying the relevant characteristics of flavonoid compounds.
· Preparing database and characterization reports for kaempferol extracts.
A1.3.
· Selecting donor-acceptor components of natural deep eutectic solvents, according to the work plan.
· Preparing deep eutectic systems by incorporating of kaempferol into the NADES structures. Database and characterization reports for natural eutectic solvents
· Obtaining and full characterization of kaempferol-NADES complexes. Database and characterization reports for kaempferol complexes with natural eutectic solvents
➢ working visits to USMF Moldova and internships at ICMPP Iasi: 10.11.-15.11.2025- working visits to USMF Moldova; 26.0-911.2025 and 17.1.2025-17.12.2025- internships to ICMPP Iasi
➢ training in the project's research area for doctoral and post-doctoral students: training on the preparation methods of kaempfrol, NADES and their complexes and characterization techniques at ICMPP during the internships of Partner Team members (Andrei Chiaburu, Ianos Coretchi) and USMF during the working visits (Mirela-Fernanda Zaltariov, Bianca-Iulia Ciubotaru, Nicoleta Vornicu, Mihaela Balan-Porcarasu)and by participating to Webinars: ″Organoids: A Key Tool for Drug Discovery″, October 16th 2025 (Bianca-Iulia Ciubotaru, Ecaterina Pavlovschi, Mirela-Fernanda Zaltariov); “From bench to bedside: emerging approaches in cancer drug discovery”, October 28th 2025 (Bianca-Iulia Ciubotaru, Ecaterina Pavlovschi, Mirela-Fernanda Zaltariov)
➢ project website: https://icmpp.ro/projects/l6/about.php?id=187
➢ dissemination of results through participation in national/international conferences: The 12th edition of the International Scientific Conference "Cultural Heritage of Yesterday – Implications for the Development of Tomorrow's Sustainable Society," Section 11 – Life Sciences, Exact and Engineering Sciences, September 18-22, 2025, Chisinau, Rep. Moldova; The International Congress “Medicine, Molecular and Environmental Sciences - MedMolMed November 10-15, 2025”, Chisinau, Rep. Moldova.
➢ 1 ISI article submitted for publication: Accelerated photodegradation of PDMS catalyzed by La(III) complexes in photoaging experiments
➢ Scientific Report.
The cognitive and socio-economic impact of scientific results
The results obtained in the 2025 stage highlighted a significant scientific impact both at the institutional level and at the level of bilateral cooperation between Romania and the Republic of Moldova, by strengthening the technical and scientific foundations necessary for the development of the subsequent stages of the project.
The experimental basis for obtaining kaempferol from local plant sources was established for the first time, contributing to the expansion of knowledge on the use of Armoracia rusticana as a raw material for bioactive compounds. The first series of kaempferol-NADES complexes were produced, opening up new directions for these formulations in the field of bionanocomposites for ophthalmic applications.
The use of natural eutectic solvents (NADES) in the development of kaempferol-based formulations represents an innovative direction in the approach to green chemistry methods with a remarkable cognitive and socio-economic impact, offering paths to safer, more sustainable technologies and promoting the development of new skills for technology transfer.
The preliminary data obtained in stage 2025 were disseminated in a preliminary form through joint participation in the 12th edition of the international scientific conference "Cultural heritage of yesterday – implications for the development of a sustainable society tomorrow", September 18-22, 2025. - Section 11 - Life Sciences, Exact and Engineering Sciences in the form of an oral presentation, but also at an international scientific event marking 35 years of Moldovan-Romanian collaboration, consolidating the visibility of the project and creating the conditions for the development of the first impactful scientific papers. Scientific mobility activities have contributed to the harmonization of experimental protocols between partners and the integration of advanced characterization methods, with team members benefiting from training in the project's research area, ensuring a high cognitive impact.
The project contributed to the development of the skills of team members from the Republic of Moldova in the field of extractive technologies, spectroscopic methods, and natural eutectic solvents. The research internships carried out at ICMPP Iași strengthened the transfer of knowledge and professional training of young researchers involved in the project. Bilateral collaboration has led to more efficient work processes and integration into international scientific networks.
The use of native plant sources to obtain bioactive compounds supports the exploitation of local natural resources promoting a high socio-economic impact and may generate, in later stages, opportunities for the development of products with potential applications in the field of health,. The project contributes to increasing research capacity in the field of biomaterials, with a long-term impact on innovation in ophthalmology and biomedicine. Dissemination activities increase the visibility of national research and promote cross-border collaboration as a tool for scientific progress.
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