The aim of the project is to develop new tailored collagen-based formulas with specific bioactive pyrrole-sulfonamide hybrids. These formulas are designed to address the pressing medical need for effective treatments for patients with chronic wounds, considered a burden on global healthcare system (affecting 1.51 to 2.21 per 1000 population) through its economic and social impact. Faster and more efficient therapeutic strategies will be highly benefic considering costs and patients’ psychological well-being. The project will build on our existing expertise on synthesis of biological active N-heterocycles and development and characterization of collagen-based compositions for biomedical applications, aiming to improve existing dressings to facilitate healing of chronic wounds. Our approach involves the fusion of recently developed pyrrole-sulfonamide hybrids, which exhibit potential antimicrobial properties, with collagen, a material known for its biocompatibility, biodegradability, low immunogenicity, and ability to promote wound healing. The advantages that differentiate the synthesis protocol for pyrrole-sulfonamide hybrids are short reaction times, clean work-up, cheap catalyst and high yields. The resulting bioactive formulas will be subjected to rigorous in vitro testing to confirm their biological activity and ability to enhance wound healing. Ultimately, this project aims to establish laboratory-scale technology for producing antimicrobial tissue regeneration formulas.