• Results of the enzyme inhibition tests- achieved objective;
• Results of encapsulation of at least two complexes into liposomes- achieved objective;
• At least one full-paper submitted at an ISI journal -achieved objective;
• Scientific Report - achieved objective.

• Results of the enzyme inhibition tests-  achieved objective

By using molecular docking studies, fluorescence and circular dichroism techniques, the ability of the obtained proligands and metal complexes in the protein binding (HSA-Human Serum Albumin) was highlighted. Molecular docking studies have shown the ability of ligands and metal complexes to inhibit Ribonucleotide Reductase (RNR), a key enzyme involved in the DNA metabolism.

Complex Docking (Receptor@Ligand)	Contact No.	Contact Receptors (R2 RNR)	Eb (kcal/mol)	Kd (µM)
R2-RNR@L1	19	GLU233, GLY234, PHE237, SER238, PHE241, SER264, ARG265, GLU267, GLY268, CYS271, ASP272, CYS275, TYR324, PHE327, VAL328, ARG331, LEU332, GLU335, ASN346	-6.181	29.462
R2-RNR@C1	17	GLU233, GLY234, PHE237, SER238, PHE241, SER264, GLU267, GLY268, CYS271, ASP272, CYS275, TYR324, PHE327, VAL328, ARG331, LEU332, GLU335	-8.393	0.704

• Results of encapsulation of at least two complexes into liposomes- achieved objective

Two copper metal complexes were encapsulated in liposomes, which previously demonstrated the highest stability in buffer at different pH and proved to be biocompatible with normal cells and to show selectivity on cancer cells. Liposomal structures based on biopolymeric surfactants were obtained in the laboratory and previously tested for the encapsulation of some drugs.

The obtained liposomal formulations were characterized in terms of dimensionality and stability (by DLS) and also were tested in vivo on rats to determine the biodistribution at i.v. of liposomes by PET-MRI imaging and post-administration animal monitoring.

• At least one full-paper submitted at an ISI journal - achieved objective

• Published articles:
• Blends of sodium deoxycholate-based poly(ester ether)urethane ionomer and hydroxypropyl cellulose with mucosal adhesiveness, Doina Macocinschi, Daniela Filip, Bianca-Iulia Ciubotaru, Raluca Petronela Dumitriu, Mirela-Fernanda Zaltariov*, International Journal of Biological Macromolecules 162, 1262-1275 (2020).

• Published Proceeding Articles:
• Cytotoxicity evaluation of new ruthenium complexes, Mirela-Fernanda Zaltariov*, Maria Butnaru, Dragos Peptanariu, 2019 E-Health and Bioengineering Conference (EHB), ISBN – 978-1-7281-2603-6, INSPEC Accession Number:19300285, DOI: 10.1109/EHB47216.2019.8969978, pp.1-4, (2020).
• Structural and cytotoxic evaluation of platinum(IV) complexes with biological active ligands, Mirela-Fernanda Zaltariov*, Mihaela Avadanei, Dragos Peptanariu, 2019 E-Health and Bioengineering Conference (EHB), ISBN – 978-1-7281-2603-6, INSPEC Accession Number: 19300423, DOI: 10.1109/EHB47216.2019.8970005, pp.1-4, (2020)
• Mucoadhesive composites based on polyurethane/AgNPs for urinary catheter application, Bianca-Iulia Ciubotaru, Mirela-Fernanda Zaltariov*, Liliana Verestiuc, Daniela Filip, Doina Macocinschi, 2019 E-Health and Bioengineering Conference (EHB), ISBN – 978-1-7281-2603-6, INSPEC Accession Number: 19315184, DOI: 10.1109/EHB47216.2019.8970045, pp.1-4, (2020).

• Book Chapters:
• Bio-based polymers for liposomal drug formulations, Mirela-Fernanda Zaltariov*, Bianca-Iulia Ciubotaru, Marcela Savin, Daniela Filip, Doina Macocinschi, Chapter 4, in Applications of Biodegradable and Bio-Based Polymers for Human Health and a Cleaner Environment, Eds. I. Stoica, O. Mukbaniani, A. K. Haghi, Apple Academic Press, partnered with CRC Press, a member of the Taylor & Francis Group, ISBN: 9781771889766, (2020).
• Metal complexes-based catalysts for oxidation reactions as new alternatives for catalytic processes in the production of bio-based polymers, Mirela-Fernanda Zaltariov*, Chapter 6, in Applications of Biodegradable and Bio-Based Polymers for Human Health and a Cleaner Environment, Eds. I. Stoica, O. Mukbaniani, A. K. Haghi, Apple Academic Press, partnered with CRC Press, a member of the Taylor & Francis Group, ISBN: 9781771889766, (2020).

• Conferences:

• Metal complexes in medicine: from drug design to therapeutic agents, Mirela-Fernanda Zaltariov, “International Congress of „Apollonia” University from Iași”-By promoting excellence, we prepare the future, Edition XXX, Iași, Romania, 27th of February – 1st of March (2020) – Invited Conference

• The results of this stage were updated on the web page of the project: http://www.icmpp.ro/metdrug

• Scientific Report- achieved objective.